Table 3

Effects of combinations of meAEA and capsaicin upon the proliferation of C6 glioma cells

CapsaicinAntagonistsCell Number (× 103 well−1) after [meAEA] (μM) of:
0110
μM
 030.9  ± 0.7028.0  ± 3.213.7  ± 3.7
 0 + 30.4  ± 3.926.0  ± 3.07.3  ± 1.9
 129.7  ± 3.427.1  ± 1.68.1  ± 1.8
 1 + 26.8  ± 3.223.4  ± 2.94.2  ± 0.67
1023.0  ± 2.824.1  ± 1.04.8  ± 1.1
10 + 23.6  ± 3.319.9  ± 2.92.4  ± 0.43

C6 glioma cells were treated daily for 4 days with the concentrations of compounds shown, as described under Experimental Procedures. Shown are means ± S.E.M., n = 4. In the absence of any solvents, the cell number was 33.4 ± 2.9 × 103 well−1. In the “Antagonists” column, − and + refer to solvent control and the combination of AM251 (0.3 μM), AM630 (0.3 μM), and capsazepine (1 μM), respectively. Two-way ANOVA of the results obtained in the absence of antagonists revealed a significant effect of both capsaicin (F2,27 = 6.26; p = 0.006) and meAEA (F2,27 = 57.47; p < 0.0001) but no interaction between the compounds (F4,27 = 0.53;p = 0.72).