[3H]-RX821002Kd25°C | PRAZOSINKi | GUANFACINE Ki | |||
---|---|---|---|---|---|
nM | nM | nM | |||
SBH | |||||
Normal salt | 1.5 ± 0.3 | 45 ± 2 | 1010 ± 160 | ||
High salt | 1.7 ± 0.4 | 48 ± 3 | 1160 ± 190 | ||
SBN | |||||
Normal salt | 1.2 ± 0.4 | 38 ± 5 (75–85%) 1920 ± 160 | 28 ± 4 (15–25%) 1560 ± 270 | ||
High salt | 1.4 ± 0.5 | 35 ± 4 (60–70%) 2300 ± 230 | 37 ± 5 (30–40%) 1580 ± 170 |
Data shown are mean ± S.E.M. of six separate experiments. Each experiment was conducted in duplicate.Kd25°C, equilibrium dissociation constant obtained from saturation curves (Fig. 1).Ki, inhibition constants obtained from competition experiments (Fig. 3). In parentheses, relative proportions of high affinity sites for each competitor.