Treatment | Dose | Wet-Dog Shake Response | ||
---|---|---|---|---|
No. of Rats Responding | No. of Shakes/Rat/h Mean ± S.E.M. | Control | ||
mg/kg | % | |||
Acute treatment | ||||
Spontaneous (before DOI) | 15 /15 | 6.3 ± 0.36 | ||
Saline | 10 /10 | 33 ± 1.9 | ||
8-OH-DPAT | 0.1 s.c. | 5 /5 | 32 ± 1.9 | |
NAE-086 | 1.0 s.c. | 5 /5 | 32 ± 3.6 | |
NAE-086 | 3.0 p.o. | 5 /5 | 32 ± 3.2 | |
Repeated treatment | ||||
Spontaneous (before DOI) | 5 /5 | 6.2 ± 0.37 | ||
Saline | 5 /5 | 33 ± 2.2 | ||
8-OH-DPAT | 2 × 0.1 s.c. | 5 /5 | 63 ± 3.48-160 | 191 |
NAE-086 | 2 × 1.0 s.c. | 5 /5 | 64 ± 4.98-160 | 194 |
NAE-086 | 2 × 3.0 p.o. | 5 /5 | 88 ± 0.68-160 | 267 |
Rats (B&K Sprague-Dawley strain) were treated twice daily for 21 days with NAE-086 or 8-OH-DPAT. The wet-dog shake response was induced by DOI (0.3 mg/kg s.c.) 24 h after the acute or last administration of the compound and 5 min before the beginning of the observation.
↵8-160 p < 0.01 vs. DOI-treated control rats (Dunnett's t test following ANOVA).