In vitro effects of alpidem but not zolpidem on ADP-supported respiration sustained by malate and glutamate
| Oxygen Consumption | RCR | ADP/O | ||
|---|---|---|---|---|
| State 3 | State 4 | |||
| nmol O/min/mg of protein | ||||
| Control | 153 ± 2 | 33 ± 1 | 4.8 ± 0.5 | 2.7 ± 0.3 |
| Alpidem (50 μM) | 112 ± 32-150 | 45 ± 2 | 2.4 ± 0.72-150 | 1.9 ± 0.1 |
| Alpidem (100 μM) | 99 ± 32-150 | 47 ± 32-150 | 2.0 ± 0.52-150 | 1.8 ± 0.12-150 |
| Alpidem (250 μM) | 72 ± 152-150 | 83 ± 92-150 | 0.8 ± 1.02-150 | 1.8 ± 0.22-150 |
| Zolpidem (250 μM) | 144 ± 14 | 38 ± 5 | 4.1 ± 0.5 | 3.1 ± 0.2 |
Mitochondria were incubated at 30°C with glutamate and malate (5 mM each). One minute after addition of alpidem or zolpidem, ADP (0.2 mM) was added and respiration was measured. Results are means ± S.E.M. for at least three experiments.
O, atom of oxygen; RCR, respiratory control ratio; ADP/O, ADP formed per O consumed during state 3 respiration.
↵2-150 Different from control (P < 0.05).