Effect of insulin treatment on NE transport and [3H]nisoxetine binding in intact SK-N-SH cells
| Pretreatment | [3H]NE Uptake | [3H]Nisoxetine Binding | ||
|---|---|---|---|---|
| Km | Vmax | Kd | Bmax | |
| nM | pmol/106 cells/min | nM | pmol/mg protein | |
| Control | 486 ± 40 | 3.61 ± 0.46 | 5.3 ± 0.37 | 0.89 ± 0.05 |
| Insulin | 451 ± 55 | 4.48 ± 0.502-150 | 5.1 ± 0.48 | 0.90 ± 0.07 |
Cells were incubated with 10 nM insulin for 60 min at 37°C. After drug treatment, uptake and binding assays in intact SK-N-SH cells were carried out as described under Materials and Methods. Km and Vmax values of transport kinetics were derived from Hofstee modification of the Michaelis-Menten equation whereas Kd and Bmaxvalues of binding assays were derived from Scatchard analysis. Data are presented as mean ± S.E.M. of three experiments carried out in triplicate.
↵2-150 Significant difference as compared with control values ( p < 0.05, Student's paired t test).