Specific biding of fractionated ligands derived from Glc-S-C8-APL17000 to rat kidney membrane
| Fraction | Elution Time3-a | Mol. wt.3-b | Binding3-c | Binding3-d(+1 μM Glc-S-AVP) | Specific3-e |
|---|---|---|---|---|---|
| min | pmol/mg of protein | ||||
| 1 | 22–24 | 16,600–33,300 | 0.891 ± 0.081 | 0.916 ± 0.103 | N.D. |
| 2 | 24–24.5 | 13,900–16,600 | 0.647 ± 0.046 | 0.594 ± 0.039 | 0.074 ± 0.060 |
| 3 | 24.5–25 | 11,700–13,900 | 0.589 ± 0.005 | 0.540 ± 0.010 | 0.049 ± 0.011 |
| 4 | 25–25.5 | 9,810–11,700 | 0.607 ± 0.023 | 0.442 ± 0.0453-150 | 0.165 ± 0.050 |
| 5 | 25.5–26 | 8,240–9,810 | 0.864 ± 0.049 | 0.504 ± 0.0493-150 | 0.361 ± 0.070 |
| 6 | 26–28 | 4,100–8,240 | 0.785 ± 0.010 | 0.368 ± 0.0353-150 | 0.416 ± 0.041 |
| 7 | 28–30 | 2,040–4,100 | 0.972 ± 0.044 | 0.514 ± 0.0613-150 | 0.458 ± 0.076 |
Fractionated ligands (2 nM) derived from Glc-S-C8-APL17000 was incubated with rat kidney membrane and the binding was determined.
N.D., not determined.
↵3-150 p < 0.05.
↵3-a Elution time in gel filtration HPLC.
↵3-b Molecular weight determined by gel filtration HPLC.
↵3-c Binding at 2 nM of the fractionated ligand alone.
↵3-d Binding at 2 nM of the fractionated ligand in the presence of 1 μM Glc-S-C8-AVP.
↵3-e Specific binding was assessed as the binding in the presence of 1 μM Glc-S-C8-AVP subtracted from that in its absence.