Complestatin attenuates neither oxidative stress nor apoptosis in cortical neurons
| Injuries | LDH Release | ||
|---|---|---|---|
| CTRL | COMP | ||
| % | |||
| Oxidative stress | Fe2+ | 100.9 ± 19.3 | 103.2 ± 13.2 |
| H2O2 | 76.9 ± 17.2 | 70.3 ± 18.6 | |
| BSO | 54.3 ± 22.2 | 77.3 ± 15.5 | |
| Apoptosis | ST | 60.2 ± 11.9 | 59.8 ± 8.1 |
| CY | 54.6 ± 8.5 | 41.6 ± 4.0 | |
Cortical cell cultures (DIV 12 to DIV 14) were exposed to oxidative stress [50 μM FeCl2, 150 μM H2O2, or 10 mM buthionine sulfoximine (BSO)] or apoptosis-inducing agents [100 nM staurosporine (ST) or 20 μM cyclosporine A (CY)], alone (CTRL) or with the addition of 3 μM complestatin (COMP). Neuronal death was analyzed by LDH assay as described above (n = 12 culture well/condition). No significant difference was observed from the relevant control.