Time course of procedures and order of testing for subjects in the current experiment
| Chronology | Test/Procedure |
|---|---|
| Presurgery days 1 to 7 | Adjustment to the colony room |
| Presurgery days 8 to 21 | Baseline EtOH intake |
| Surgical Pump Implantation | |
| Postsurgery days 1 to 12 | EtOH intake |
| Postsurgery day 13 | No testing |
| Postsurgery days 14 to 16 | Saccharin preference |
| Postsurgery day 17 | No testing |
| Postsurgery days 18 to 21 | Open field behaviors |
| Postsurgery day 22 | No testing |
| Postsurgery day 23 | Elevated plus maze |
| Postsurgery days 24 to 26 | Additional group specific testing |
| Postsurgery day 26 | Sacrifice and brain removal for functional receptor binding |
The table summarizes daily testing schedule and overall chronology of subjects treated chronically with 5-HT2A antisense given i.c.v. or targeted to the specific brain areas listed.