Gene-specific PCR primers
| Gene1-a | Forward Primer1-b | Reverse Primer1-b | Base Pair1-c |
|---|---|---|---|
| MDR1 (ABCB1) | 5′-CTCATCGTTTGTCTACAGTTCGT | 5′-GCTTTCTGTCTTGGGCTTGT | 1873–2160 |
| MDR3 (ABCB4) | 5′-TGGAGCAAGGAAGCCACAG | 5′-TGGCAATGGCACATACTGTT | 1858–2193 |
| ABCB5 | 5′-GACATCGGCTGGTTTGATAGCT | 5′-GAGTTTCCAGCCCTTCACCA | 57103–57355 |
| MRP1 (ABCC1) | 5′-CATGAAGGCCATCGGACTCT | 5′-CAGGTCCACGTGCAGACA | 3094–3352 |
| MRP2 (ABCC2) | 5′-GACTATGGGCTGATATCCAGTGT | 5′-AGGCACTCCAGAAATGTGCT | 2687–3176 |
| MRP3 (ABCC3) | 5′-GGCACTGCTGATTGAAGACA | 5′-AATGGCTGCTTTCTCCTCCT | 2639–2867 |
| MRP4 (ABCC4) | 5′-AAGTGAACAACCTCCAGTTCCA | 5′-CCGGAGCTTTCAGAATTGAC | 2026–2543 |
| MRP5 (ABCC5) | 5′-CATGCTGATCCTGAAAGCCAT | 5′-GAATCAGGACCCTGGAGACA | 2968–3305 |
| MRP6 (ABCC6) | 5′-TCAGAAGCCCAGACAGAGGT | 5′-CCCAGCGTAGAGGAGAAACA | 2704–3300 |
| BCRP (ABCG2) | 5′-TTTCAGCCGTGGAACTCTTT | 5′-TGAGTCCTGGGCAGAAGTTT | 1563–2024 |
| LRP (MVP) | 5′-CAACTGGCACTTTGAGGTGA | 5′-CTGGGAGTTGGTGGTGATCT | 1734–2112 |
| CYP3A4 | 5′-AGAAAGTCGCCTCGAAGATACAC | 5′-TGCAGTTTCTGCTGGACATC | 780–1004 |
| Villin 1 (VIL1) | 5′-AAAGCCAATGAGCAGGAGAA | 5′-ATGGATGTGGCATCGAACTT | 928–1163 |
↵1-a Name within parentheses or single name corresponds to the Homo sapiens Official Gene Symbol, according to the HUGO Gene Nomenclature Committee (http://www.gene.ucl.ac.uk/nomenclature/).
↵1-b An additional ACTGGATCC sequence was incorporated at the 5′-end of both the forward and the reverse primer.
↵1-c Area amplified refers to sequences deposited at GenBank with accession numbers as follows: AF016535(MDR1), M23234 (MDR3), AC002486(ABCB5), L05628 (MRP1), U63970 (MRP2),AB010887 (MRP3), AF071202 (MRP4), AF146074(MRP5), AF168791 (MRP6), AF098951(BCRP), X79882 (LRP), AF182273(CYP3A4), and X12901 (Villin).