Treatment | Apoptosis | |
---|---|---|
BxPC-3 | PaCa-2 | |
% | ||
Control (DMSO) | 0.9 | 0.3 |
Sulindac | ||
250 μM | 0.3 | 0.8 |
500 μM | 7.9 | 8.2 |
Indomethacin, 100 μM | 1.1 | 2.2 |
NS-398, 100 μM | 1.5 | 0.2 |
Gemcitabine, 20 nM | 0.4 | 0.5 |
Extent of apoptosis following treatment of BxPC-3 and PaCa-2 cells with the indicated NSAIDs or gemcitabine for 3 days was measured by the incorporation of FITC-dUTP in the presence of TdT enzyme to detect DNA fragmentation. Cells were stained with RNase A/PI and analyzed by flow cytometry. The results from a representative experiment are presented, and similar results were obtained in at least two separate experiments.