The effect of OKA on tyrosine hydroxylase and trypan blue-positive cells
| Condition | TH Positive | Trypan Blue Positive |
|---|---|---|
| Control | 158 ± 22 | 32 ± 2 |
| OKA | 72 ± 19 | 211 ± 23 |
Mesencephalic cultures were treated with 5 nM OKA for 24 h followed by 3 days of recovery. Cultures were immunostained for TH or stained for viability with trypan blue and 10, 1-mm2 fields were counted as described under Materials and Methods. Duplicate conditions per experiment in three experiments were analyzed. Values are presented as mean number of cells/mm2 ± S.E.M.