In vitro blood cell association and stability of [125I]SB-251353
| GROβ-T | RANTES | Time at 37°C | Blood/Plasma Conc. Ratio2-a2-b | Percent Cell Association2-a2-c | Percent TCA Precipitable2-d |
|---|---|---|---|---|---|
| ng/ml | μg/ml | min | |||
| 50 | 0 | 02-e | 0.875 (0.020) | 21.67 (5.10) | 87.0 |
| 25 | 02-e | 0.616* | N.D. | N.D. | |
| 0 | 10 | 0.827 (0.028) | 16.72 (0.70) | N.D. | |
| 0 | 60 | 0.739 (0.040) | 17.42 (2.87) | 88.6 | |
| 25,000 | 0 | 02-e | 0.617 (0.031) | 1.69 (0.55) | N.D. |
| 0 | 10 | 0.589 (0.013) | 1.27 (0.21) | N.D. | |
| 0 | 60 | 0.592 (0.040) | 1.86 (0.26) | N.D. |
↵2-a Mean (standard deviation);n = 3 except for * where n = 2 (no standard deviation reported).
↵2-b Hematocrit of pooled mouse whole blood was 0.43.
↵2-c Radioactivity remaining in the cell pellet after three sequential phosphate-buffered saline washes.
↵2-d Percentage of radioactivity in plasma, pooled from whole blood incubation, that was trichloroacetic acid (TCA) precipitable (N.D. indicates no data).
↵2-e Incubation performed on wet ice (∼4°C).