Kinetic parameters in MCF7, BC19, and PSC 833-treated BC19 (PSC-BC19) cells
| MCF7 | BC19 | PSC-BC19 | |
|---|---|---|---|
| B max,c(μM) | 59.3 ± 3.042-a | —2-b | 61.5 ± 7.33 |
| K d,c (nM) | 4.93 ± 0.7462-a | —2-b | 5.24 ± 1.61 |
| NSB | 0.148 ± 0.0282-a | —2-b | 0.118 ± 0.055 |
| CLf,d (× 10−3μl/h/cell) | 3.34 ± 0.3172-a | —2-b | 3.62 ± 0.493 |
| J max (× 10−4pmol/h/cell) | —2-b | 2.81 ± 0.25 | —2-b |
| K M,Pgp(nM) | —2-b | 13.9 ± 4.36 | —2-b |
| Initial C total,m (nM) | |||
| k cell number(h−1) | |||
| 1 | 0.00882-a | 0.0075 | 0.097 |
| 10 | 0.00462-a | 0.0041 | 0.0051 |
| 100 | −0.00532-a | −0.0042 | −0.0047 |
| 1000 | −0.00532-a | −0.0063 | −0.0042 |
| kB max,c(× 10−3 h−1)2-a | |||
| 1 | 2.47 ± 0.1522-c | ||
| 10 | 5.17 ± 0.2082-c | ||
| 100 | 10.8 ± 2.082-c | ||
| 1000 | 31.3 ± 4.252-c |
The definition of the model parameters and their values were as described under Materials and Methods. ForBmax,c, Kd,c, NSB, CLf,d,Jmax, and KM,Pgp, the results represent the mean ± S.D. for the values determined from analyzing the four profiles obtained for four initial extracellular drug concentrations (i.e., 1, 10, 100, and 1000 nM). Forkcell number andkBmax,c, the values obtained for the four initial extracellular drug concentrations are provided. The extracellular binding constants to proteins in the culture medium are not cell-type specific and, as determined previously, are 3.94 μM forBmax,m and 781 nM for Kd,m (Kuh et al., 2000).