Table 3

Kinetic parameters for N-demethylation of LAAM and nor-LAAM by human intestinal microsomes

	nor-LAAM from LAAM		dinor-LAAM from LAAM		dinor-LAAM from nor-LAAM
	K_m1	V_max1	V_max1/K_m1	K_m2	V_max2	K_m1	V_max1	V_max1/K_m1	K_m2	V_max2	K_m1	V_max1	V_max1/K_m1	K_m2	V_max2
	μM	nmol/min/mg	ml/min/mg ×10⁻³	μM	nmol/min/mg	μM	nmol/min/mg	ml/min/mg ×10⁻³	μM	nmol/min/mg	μM	nmol/min/mg	ml/min/mg ×10⁻³	μM	nmol/min/mg
HIM 28	25	0.7	26	750	2.1	11	0.006	0.51	740	0.02	25	0.2	8.8	940	0.5
HIM 31	20	0.4	21	1230	2.2	9	0.003	0.34	640	0.01	10	0.1	7.4	1040	1.0
HIM 40	19	0.3	16	960	2.2	13	0.004	0.28	1020	0.02	20	0.1	5.3	1490	1.4

Kinetic parameters were obtained using a dual-enzyme Michaelis-Menten model.