Table 2

Acute ethanol inhibition of D2L receptor coupling is abolished by chronic exposure to ethanol

	cAMP Production − Acute Ethanol		% Inhibition of cAMP by NPA	cAMP Production ⁺ Acute ethanol		% Inhibition of cAMP by NPA
	−NPA	⁺NPA	% Inhibition of cAMP by NPA	−NPA	⁺NPA	% Inhibition of cAMP by NPA
Control	968 ± 47	629 ± 51	35 ± 3	1402 ± 108	1318 ± 110	6 ± 1^2-160
Chronic ethanol	774 ± 50	526 ± 32	32 ± 2	810 ± 29	518 ± 22	36 ± 5

NG108-15 cells were incubated in the presence or absence of 150 mM ethanol for 72 h. The media were then changed and cells incubated for an additional 30 min in the presence or absence of 200 mM ethanol and NPA as described in Fig. 1 and inhibition of PGE₁-stimulated cAMP production measured (pmol/mg of protein). Data shown are the means ± S.E. n = 5.

↵2-160 p < 0.01, compared with NPA inhibition of PGE₁-stimulated cAMP in the control cells (one way analysis of variance and Dunnett's test).