Table 1

Kd and Bmax values of HEK293 cells stably expressing the human ORL1 receptor after different treatments

TreatmentKdBmax
pM fmol/mg
Control cells71  ± 12 (n = 5)1205  ± 52 (n = 5)
OFQ/N desensitized (pH 7.4-wash)301  ± 91-a (n = 4)865  ± 111-b (n = 4)
OFQ/N desensitized (pH 4.0-wash)72  ± 6 (n = 4)1213  ± 83 (n = 4)
Ro-64-6198 desensitized (pH 4.0-wash)351  ± 131-a (n = 5)741  ± 651-c (n = 5)

The data are means ± S.E.M. from at least three different binding experiments performed in triplicate.

  • Statistically significant differences:

  • ↵1-a  p < 0.0001 versus control cells and OFQ/N desensitized (pH 4.0-wash);

  • ↵1-b  p < 0.001 versus control cells and OFQ/N desensitized (pH 4.0-wash);

  • ↵1-c  p < 0.0001 versus control cells and OFQ/N desensitized (pH 4.0-wash).