Effect of methamphetamine on dorsal brainstem monoamine and metabolite levels of adult mice exposed to the drug in utero
| Sex | Prenatal Exposure | Challenge | DA | DOPAC | HVA | 5-HT | 5-HIAA |
|---|---|---|---|---|---|---|---|
| Male | Meth (4) | Saline | 1.0 ± 0.1 | N.D. | 1.0 ± 0.1 | 8.8 ± 0.3 | 6.3 ± 0.5 |
| Meth (4) | 5 mg/kg | 0.7 ± 0.1 | N.D. | 0.7 ± 0.1 | 7.7 ± 0.2 | 5.4 ± 0.3 | |
| Meth (4) | 10 mg/kg | 0.8 ± 0.1 | N.D. | 0.8 ± 0.1 | 8.3 ± 0.4 | 5.4 ± 0.4 | |
| Meth (4) | 15 mg/kg | 0.7 ± 0.1 | N.D. | 0.7 ± 0.2 | 8.5 ± 0.3 | 5.6 ± 0.1 | |
| Meth (3) | 20 mg/kg | 0.6 ± 0.1 | N.D. | 0.6 ± 0.0 | 8.0 ± 0.3 | 5.9 ± 0.2 | |
| Male | Saline (4) | Saline | 0.9 ± 0.1 | N.D. | 0.9 ± 0.1 | 8.1 ± 0.3 | 5.8 ± 0.3 |
| Saline (4) | 5 mg/kg | 0.9 ± 0.2 | N.D. | 0.8 ± 0.3 | 7.8 ± 0.8 | 5.5 ± 0.3 | |
| Saline (4) | 10 mg/kg | 0.7 ± 0.0 | N.D. | 0.7 ± 0.1 | 8.1 ± 0.2 | 5.5 ± 0.2 | |
| Saline (3) | 15 mg/kg | 0.7 ± 0.1 | N.D. | 0.7 ± 0.0 | 8.7 ± 1.0 | 5.6 ± 0.2 | |
| Saline (3) | 20 mg/kg | 0.6 ± 0.1 | N.D. | 0.6 ± 0.0 | 7.9 ± 0.1 | 5.8 ± 0.1 | |
| Female | Meth (4) | Saline | 0.9 ± 0.1 | N.D. | 0.9 ± 0.1 | 8.0 ± 0.5 | 7.6 ± 0.5 |
| Meth (4) | 5 mg/kg | 1.0 ± 0.1 | N.D. | 0.9 ± 0.0 | 8.2 ± 0.5 | 7.7 ± 0.2 | |
| Meth (4) | 10 mg/kg | 0.7 ± 0.1 | N.D. | 0.7 ± 0.1 | 7.9 ± 0.4 | 7.4 ± 0.4 | |
| Meth (4) | 15 mg/kg | 0.7 ± 0.1 | N.D. | 0.7 ± 0.0 | 8.4 ± 0.2 | 7.9 ± 0.4 | |
| Meth (4) | 20 mg/kg | 0.7 ± 0.1 | N.D. | 0.7 ± 0.1 | 8.6 ± 0.3 | 8.2 ± 0.3 | |
| Female | Saline (4) | Saline | 1.0 ± 0.1 | N.D. | 0.9 ± 0.0 | 8.3 ± 0.2 | 8.0 ± 0.5 |
| Saline (4) | 5 mg/kg | 1.0 ± 0.2 | N.D. | 1.0 ± 0.2 | 8.3 ± 0.7 | 7.7 ± 0.5 | |
| Saline (4) | 10 mg/kg | 0.7 ± 0.1 | N.D. | 0.7 ± 0.1 | 7.2 ± 0.5 | 7.2 ± 0.3 | |
| Saline (4) | 15 mg/kg | 0.8 ± 0.0 | N.D. | 0.8 ± 0.1 | 8.1 ± 0.4 | 7.8 ± 0.2 | |
| Saline (4) | 20 mg/kg | 0.9 ± 0.2 | N.D. | 0.9 ± 0.3 | 8.4 ± 0.5 | 8.2 ± 0.8 |
Three-month-old postnatal offspring, exposed in utero to saline or methamphetamine, were challenged with two doses of saline or methamphetamine (5, 10, 15, or 20 mg/kg/dose) and killed 7 days later. Data represent the mean ± S.E.M. of dorsal brainstem monoamine and metabolite levels (ng/mg of protein) of individual animals from three to four litters (n) per treatment group.
N.D., nondetectable.