ODN 32/50 nM | ODN 83/50 nM | Cisplatin | ||
---|---|---|---|---|
0 | 40 μM | |||
Experiment 1 | ||||
Proliferation rate1-a | 100 | 16.771-150 | ||
Apoptosis1-b | 6.98 | 7.13 | 0 | 85.44 |
Experiment 2 | ||||
Proliferation rate | 100 | 8.561-150 | ||
Apoptosis | 3.10 | 3.47 |
HeLa cells were treated with control scrambled ODN 32, TS antisense ODN 83, or cisplatin, and assayed for apoptosis after 15 h as described under Materials and Methods.
↵1-150 Significant difference from ODN 32-treated cells (p < 0.05, Student's t test).
↵1-a Proliferation rate relative to ODN 32-treated cells was determined in each experiment from cell numbers in control flasks, and calculated using the formula:
100 × [(final cell # ÷ starting cell #) − 1] ÷ [(ODN 32 final cell # ÷ starting cell #) − 1]
↵1-b Percentage of apoptotic cells in each condition.