Information on human liver microsomal preparations used in the study
| Parameter Measured | Male Donors | Female Donors |
|---|---|---|
| Number of donors | 21 | 12 |
| Age of donors (yr) | 47 ± 3 | 56 ± 16 |
| Adjusted protein content (mg/ml) | 20 | 20 |
| Total CYP content1-a | 0.45 ± 0.29 | 0.43 ± 0.20 |
| Cytochrome b5content1-b | 0.46 ± 0.12 | 0.38 ± 0.13 |
| NADPH-cytochrome c reductase1-b | 205.3 ± 63.2 | 195.9 ± 53.3 |
| Caffeine N 3-demethylation (1A2)1-c | 47.0 ± 24.2 | 33.1 ± 50.5 |
| Coumarin 7-hydroxylation (2A6)1-c | 1769.8 ± 1878.9 | 1470.6 ± 1283.3 |
| S -Mephenytoin N -demethylation (2B6)1-c | 136.6 ± 210.9 | 107.6 ± 111.6 |
| Tolbutamide methyl-hydroxylation (2C9)1-c | 291.7 ± 216.1 | 213.4 ± 86.0 |
| S -Mephenytoin 4′-hydroxylation (2C19)1-c | 73.7 ± 130.3 | 93.4 ± 133.2 |
| Dextromethorphan O -demethylation (2D6)1-c | 255.0 ± 171.8 | 125.3 ± 97.0 |
| Chlorzoxazone 6-hydroxylation (2E1)1-c | 2202.1 ± 601.3 | 1648.0 ± 846.9 |
| Dextromethorphan N -demethylation (3A4)1-c | 115.1 ± 194.9 | 109.2 ± 101.9 |
| Testosterone 6β-hydroxylation (3A4/5)1-c | 3963.5 ± 4951.6 | 4981.2 ± 3944.7 |
| Lauric acid 12-hydroxylation (4A11)1-c | 1145.2 ± 524.1 | 927.4 ± 477.1 |
The contents of total CYP, b5, and cytochrome c reductase and the activities for various CYP isoforms were determined according methods described earlier (Pearce et al., 1996).