Effect of pCPT-cGMP on riboflavin uptake in BeWo cell monolayers3-a
| Inhibitor | [3H]Rf Uptake (% of Control) | [14C]Ma Uptake (% of Control) |
|---|---|---|
| PKG pathways | ||
| pCPT-cGMP (250 μM) | 76.88 ± 5.143-165 | 109.24 ± 16.75 |
| pCPT-cGMP (500 μM) | 73.44 ± 4.643-160 | 101.50 ± 7.59 |
| pCPT-cGMP (250 μM) + IBMX (2.5 mM) | 43.28 ± 6.213-165 | 84.73 ± 12.14 |
| IBMX (2.5 mM) | 51.07 ± 6.313-165 | 94.38 ± 15.28 |
↵3-160 p < 0.01,
↵3-165 p < 0.001 versus untreated control.
↵3-a 5 nM [3H]riboflavin (Rf) and 0.37 μM [14C]mannitol (Ma) were added to confluent BeWo cells pretreated with these inhibitors (1 h, 37°C). Cells were washed twice with ice-cold acidic PBS, lysed with 1% Triton X-100, and measured for radioactivity after a 20-min uptake study. Each value represents the mean ± S.D. of four experiments.