Group | Normal, Vehicle Control n = 10 | FasL control (4 μg/mouse, i.v.)n = 15 | FasL (4) + TR6-Fc (93 μg/mouse, i.v.)n = 15 |
---|---|---|---|
Apoptosis score | 0.1 ± 0.1*** | 3.9 ± 0.07 | 1.3 ± 0.25*** |
Histologic evaluation was made of livers from mice injected with 1) cross-linked FasL, 4 μg/mouse, i.v.; 2) TR6-Fc, 93 μg/mouse, i.v., 1 h before FasL; and 3) normal vehicle-injected controls. One to 2 h after FasL injection, liver tissue was removed, fixed in 10% neutral-buffered formalin, processed, embedded, and sectioned by routine methods, and stained with hematoxylin and eosin for light microscopy. The results were expressed as the mean of the grading scale for each group, where 0 = <1 apoptotic cell per five 10× fields; 1 = 1 apoptotic cell per two to five 10× fields; 2 = 1 to 4 apoptotic cells per one 10× field; 3 = 5 to 20 apoptotic cells per one 10× field; and 4 = >20 apoptotic cells per one 10× field. Data are expressed as mean ± S.E.M.*** p < 0.001 compared with FasL control.