Agents | Colonies | |
---|---|---|
Serum-Containing | Serum-Attenuated | |
Control | 0 | 0 |
IL-3 | 23 ± 1 | 2 ± 1 |
TPO | 1 ± 1 | 0 |
KL | 2 ± 2 | 0 |
IL-3 + TPO | 45 ± 5a | 9 ± 22-b |
IL-3 + KL | 39 ± 5a | 27 ± 52-b |
KL + TPO | 24 ± 52-c | 4 ± 3 |
IL-3 + KL +TPO | 45 ± 32-d | 43 ± 72-e |
Day-2 post 5-FU marrow cells (5 × 104) were incubated with cytokines for 14 days in the presence of 2 units/ml EPO. Colonies were counted on day 14 of culture. Data represent mean ± S.D. of four dishes.