Condition | Cyclic AMP Accumulation | |||
---|---|---|---|---|
Pretreatment 2 h | Pretreatment 18 h | |||
Vehicle | Quinpirole (1 μM) | Vehicle | Quinpirole (1 μM) | |
pmol/well | ||||
Basal | 6.3 ± 1.6 | 7.4 ± 2.1 | 5.8 ± 1.3 | 7.8 ± 1.8 |
PMA (100 nM) | 79.4 ± 15 | 169 ± 302-150 | 104 ± 21 | 264 ± 462-150 |
Isoproterenol (1 μM) | 11.8 ± 3.2 | 10.6 ± 2.6 | 15.4 ± 3.4 | 14.2 ± 3.0 |
Forskolin (10 μM) | 360 ± 73 | 380 ± 84 | 450 ± 89 | 480 ± 87 |
A23187 (10 μM) | 6.8 ± 2.5 | 5.8 ± 2.5 | 11.8 ± 4.7 | 11.6 ± 4.3 |
ACII/D2L cells were pretreated for 2 or 18 h with vehicle or quinpirole (1 μM). Following agonist pretreatment, cells were washed extensively and cyclic AMP accumulation was stimulated under the conditions indicated for 15 min. Data shown are mean ± S.E. of 7 to 10 independent experiments.
↵2-150 p < 0.05 compared with vehicle-treated cells (Student's paired t test).