Table 1

Treatment with WR-1065 protects murine embryo fibroblasts from paclitaxel-induced cell death in a p53-dependent manner

Cell LineIC50 Values
Paclitaxel AlonePaclitaxel + 1 mM WR-1065
10.1 (p53 −/− MEF)59  ± 810  ± 5
12.1 (wt p53 MEF)54  ± 9180  ± 14
CaCl (wt p53 melanoma)2.5  ± 0.61  ± 0.5
CaCl/E6 (p53 −/− melanoma)6  ± 0.73  ± 0.8

WR-1065 does not protect the human tumor cell line CaCl from the cytotoxic effects of paclitaxel (Taxol). IC50 values for paclitaxel were calculated using the sulforhodamine B assay; numbers are in nanomolar (nM) and are the average of three independent experiments ± S.E.M.