Compound | Parameter | Units | V791-a | V79, with 50 mM NH4Cl1-b | EMT61-c |
---|---|---|---|---|---|
DACA | K 1 | 177 ± 32 | 126 ± 13 | 195 ± 14 | |
K2 | μM | 2.31 ± 0.26 | 0 | 2.27 ± 0.13 | |
Bmax | μM | 572 ± 22 | 0 | 578 ± 12 | |
kmet | min−1 | 2.55 ± 0.54 | N.D. | 0 | |
DAPA1-d | K1 | 78 ± 12 | 78 | N.D. | |
K2 | μM | 19.8 ± 4.1 | 0 | N.D. | |
Bmax | μM | 8100 ± 430 | 0 | N.D. |
Values are mean ± S.E. for fit determined by nonlinear regression (eqs. 2-7) for binding, and linear regression for metabolism (eq. 8). The parameter values are scaled to give the intracellular values using the intracellular volume fraction (φi) of the cell suspensions assuming a cell volume of 1 μl/106 cells.