Table 1

Intracellular binding and metabolism parameters for DACA and DAPA estimated from data in aerobic stirred single cell suspensions at 37°C (ca. 10⁶ cells/ml)

Compound	Parameter	Units	V79^1-a	V79, with 50 mM NH₄Cl^1-b	EMT6^1-c
DACA	K ₁		177 ± 32	126 ± 13	195 ± 14
	K₂	μM	2.31 ± 0.26	0	2.27 ± 0.13
	B_max	μM	572 ± 22	0	578 ± 12
	k_met	min⁻¹	2.55 ± 0.54	N.D.	0
DAPA^1-d	K₁		78 ± 12	78	N.D.
	K₂	μM	19.8 ± 4.1	0	N.D.
	B_max	μM	8100 ± 430	0	N.D.

Values are mean ± S.E. for fit determined by nonlinear regression (eqs. 2-7) for binding, and linear regression for metabolism (eq. 8). The parameter values are scaled to give the intracellular values using the intracellular volume fraction (φ_i) of the cell suspensions assuming a cell volume of 1 μl/10⁶ cells.

N.D., not determined.
↵1-a n = 18, R = 0.998.
↵1-b n = 15, R = 0.991.
↵1-c n = 45, R = 0.998.
↵1-d Data from Hicks et al. (1997).