Table 1

Comparison of the thermodynamic parameters determined for CV-N binding with glycoproteins

GlycoproteinKdΔHΔSΔG1-aN
μM kcal/mol eu kcal/mol CV-N/protein
gp1201-b 0.037  ± 0.003−20.20  ± 0.42−33.8  ± 1.4−10.14  ± 0.054.850  ± 0.050
gp411-b 0.606  ± 0.182−14.30  ± 1.70−19.50  ± 5.70−8.5  ± 0.20.920  ± 0.090
RNase B0.602  ± 0.093−30.95  ± 0.99−75.0  ± 3.3−8.50  ± 0.090.345  ± 0.009
RNase B (Man-8 + Man-9)0.585  ± 0.095−30.70  ± 1.00−74.3  ± 3.4−8.52  ± 0.090.991  ± 0.025
Soybean agglutinin (tetramer)0.075  ± 0.053−42.30  ± 2.20−107.0  ± 7.2−9.88  ± 0.352.910  ± 0.110
Soybean agglutinin (monomer)0.223  ± 0.115−49.60  ± 3.30−133.0  ± 10.5−9.22  ± 0.270.746  ± 0.037

  • eu, entropy unit (cal/mol · K); N, stoichiometry.

  • ↵1-a  ΔG = −RTlnK.

  • ↵1-b  O'Keefe et al. (2000).