Ex vivo effect of stavudine on [U-14C]palmitic acid β-oxidation in liver mitochondria from mice fasted for 48 h
| Acid-Soluble β-Oxidation Products | |||
|---|---|---|---|
| Total Activity (without KCN) | Mitochondrial Activity (KCN-Inhibitable) | Peroxisomal Activity (KCN-Insensitive) | |
| nmol · mg protein−1 · min−1 | |||
| Controls (n = 8) | 11.7 ± 2.2 | 8.9 ± 2.1 | 2.8 ± 0.3 |
| Stavudine (n = 8) | 16.7 ± 2.92-150 | 13.1 ± 2.82-150 | 3.6 ± 0.7 |
Lean mice were treated with stavudine (100 mg/kg/day) for 6 weeks and fasted for the last 48 h. Liver mitochondria were isolated and incubated at 30°C for 10 min with [U-14C]palmitate, ATP, coenzyme A, and carnitine in the presence or absence of 2 mM KCN. The mitochondrial β-oxidation activity was assessed as the difference between the activity measured without KCN (total activity) and that with KCN (peroxisomal activity). Results are means ± S.E.M. for eight control and eight treated mice.
↵2-150 Significant difference between control and treated mice (P < 0.05).