Table 1

The effect of glutathione-modulating agents (depletion/recycling) on cytokines (ng/mg of protein)

BSOBCNUPDTCEbselen
IL-1β
 Control4.41  ± 0.174.68  ± 0.253.72  ± 0.213.52  ± 0.29
 Variable10.89  ± 0.381-152 4.65  ± 0.233.86  ± 0.242.75  ± 0.15
 H2O2 8.11  ± 0.271-151 10.36  ± 0.421-151 9.21  ± 0.311-151 5.63  ± 0.241-150
 Variable + H2O2 11.53  ± 0.311-151 4.75  ± 0.321-154 4.22  ± 0.181-154 3.97  ± 0.261-154
 X/XO7.02  ± 0.281-150 9.22  ± 0.281-151 8.83  ± 0.221-151 7.04  ± 0.371-150
 Variable + X/XO9.88  ± 0.251-152 1-154 4.63  ± 0.291-154 3.89  ± 0.251-154 2.62  ± 0.191-154
IL-6
 Control4.21  ± 0.194.38  ± 0.184.01  ± 0.243.67  ± 0.20
 Variable7.72  ± 0.341-152 4.32  ± 0.113.96  ± 0.133.56  ± 0.17
 H2O2 7.48  ± 0.211-151 8.62  ± 0.371-151 9.06  ± 0.351-151 7.60  ± 0.211-151
 Variable +H2O2 9.44  ± 0.251-151 1-154 4.35  ± 0.241-154 4.47  ± 0.231-154 3.32  ± 0.221-154
 X/XO8.38  ± 0.271-151 10.43  ± 0.461-152 8.52  ± 0.291-151 6.33  ± 0.251-150
 Variable + X/XO9.74  ± 0.301-152 1-154 4.34  ± 0.211-154 4.06  ± 0.201-154 3.52  ± 0.161-154
TNF-α
 Control3.84  ± 0.214.01  ± 0.194.27  ± 0.183.15  ± 0.28
 Variable6.10  ± 0.321-152 3.49  ± 0.134.18  ± 0.123.13  ± 0.17
 H2O2 6.93  ± 0.251-150 7.12  ± 0.241-150 7.99  ± 0.261-150 4.70  ± 0.191-151
 Variable +H2O2 7.08  ± 0.241-150 3.53  ± 0.201-154 4.67  ± 0.201-154 1.93  ± 0.121-150 1-154
 X/XO7.42  ± 0.261-151 8.30  ± 0.231-152 8.18  ± 0.231-151 5.16  ± 0.201-150
 Variable + X/XO8.84  ± 0.331-152 1-154 3.31  ± 0.121-154 4.15  ± 0.191-154 2.62  ± 0.161-154

Data are presented as means ± S.E.M. (n = 4, each).

  • BSO, 50 μM; BCNU, 100 μM; PDTC, 100 μM; ebselen, 100 μM; H2O2, 250 μM; X/XO, 100 μM/2 mU/ml.

  • 1-150P < 0.05,

  • 1-151P < 0.01,

  • 1-152P < 0.001, as compared with control;

  • 1-154P < 0.01, as compared with H2O2 or X/XO.