Table 1

Effects of neutralization and zinc chelation on type A activity at the mouse neuromuscular junction

Treatment (n ≥ 3 Tissues)Paralysis1-a Times
min
Toxin1-b 33.35  ± 1.75
MAH1-b 1-c
Toxin +MAH73.33  ± 9.281-d
EDTA +TPEN1-b 1-c
Toxin + EDTA +TPEN81.33  ± 3.281-d

  • ↵1-a  Paralysis defined as a 90% reduction in twitch response.

  • ↵1-b  Toxin = 10−8 M; MAH = 25 mM; EDTA = 200 μM; TPEN = 15–20 μM.

  • ↵1-c  Tissues treated with MAH or chelators without toxin did not paralyze.

  • ↵1-d  Statistically significant increase in the length of time necessary to produce paralysis (P< 0.01).