Treatment (n ≥ 3 Tissues) | Paralysis1-a Times |
---|---|
min | |
Toxin1-b | 33.35 ± 1.75 |
MAH1-b 1-c | |
Toxin +MAH | 73.33 ± 9.281-d |
EDTA +TPEN1-b 1-c | |
Toxin + EDTA +TPEN | 81.33 ± 3.281-d |
↵1-a Paralysis defined as a 90% reduction in twitch response.
↵1-b Toxin = 10−8 M; MAH = 25 mM; EDTA = 200 μM; TPEN = 15–20 μM.
↵1-c Tissues treated with MAH or chelators without toxin did not paralyze.
↵1-d Statistically significant increase in the length of time necessary to produce paralysis (P< 0.01).