Table 1

LPS induces adhesion molecule expression on HL-60 and ECV304 cells

	Relative Fluorescence Units
	LFA-1	MAC-1	ICAM-1	E-selectin
HL-60
LPS (1 h)	123 ± 9^1-150	226 ± 1^1-150	N.D.	N.D.
LPS (4 h)	120 ± 11^1-150	217 ± 8^1-150	N.D.	N.D.
LPS (8 h)	131 ± 12^1-150	145 ± 9^1-150	N.D.	N.D.
ECV304
LPS (1 h)	N.D.	N.D.	121 ± 6^1-150	276 ± 11^1-150
LPS (4 h)	N.D.	N.D.	116 ± 9^1-150	243 ± 7^1-150
LPS (8 h)	N.D.	N.D.	169 ± 11^1-150	219 ± 9^1-150
HL-60/ECV304
LPS (1 h)	156 ± 11^1-150	248 ± 9^1-150	143 ± 7^1-150	342 ± 10^1-150 ^1-160
LPS (4 h)	176 ± 12^1-150	283 ± 13^1-150 ^1-160	201 ± 13^1-150 ^1-160	316 ± 11^1-150 ^1-160
LPS (8 h)	185 ± 11^1-150 ^1-160	285 ± 9^1-150 ^1-160	258 ± 12^1-150 ^1-160	263 ± 12^1-150

HL-60 (1 × 10⁶ cells/ml), ECV304 (1 × 10⁶ cells/25-cm² culture dish), or HL-60/ECV304 coculture in the above-described conditions were incubated with LPS (1 μg/ml). Adhesion molecule expression was measured as described underMaterials and Methods. Values are mean ± S.E.M. of six determinations from two experiments.

N.D., not determined.
↵1-150 P < 0.05 versus nonstimulated cultures.
↵1-160 P < 0.05 versus adhesion molecule expression by HL-60 or ECV304 cultures.