Additions | Active Free MMP-9 | Total Free MMP-9 |
---|---|---|
μM | ng/ml medium ± S.D. | |
No addition | 2.45 ± 0.01 | 2720 ± 68 |
Lacidipine (10) | 1.60 ± 0.012-150 | 1968 ± 382-150 |
Lacidipine (20) | 0.90 ± 0.032-150 | 1760 ± 872-150 |
Human macrophages were incubated has described in Table 1. Then media were collected and the amount of active and total free MMP-9 released by cells was measured by ELISA, as described under Materials and Methods. Data are the mean ± S.D. of two experiments performed in triplicate.
↵2-150 P < 0.05 versus control (not treated cells).