Km | Vmax | |||||
---|---|---|---|---|---|---|
PUT | SPD | SPM | PUT | SPD | SPM | |
μM | pmol/106 cells/min | |||||
Control | 6.9 | 4.4 | 1.3 | 57.5 | 47.4 | 26.2 |
AGM-01-a | 54.61-150 | 72.91-150 | 42.21-150 | 52.9 | 45.8 | 27.7 |
AGM 2 h1-b | 6.9 | 12.1 | 4.7 | 47.11-150 | 45.9 | 25.0 |
AGM 24 h1-b | 29.21-150 | 2.6 | 2.6 | 45.01-150 | 22.61-150 | 23.9 |
↵1-150 Different from control at p < 0.05.
↵1-a Agmatine (1 mM) was present in the culture medium during the 30-min polyamine uptake determination.
↵1-b Agmatine (1 mM) was added to the culture medium for the indicated durations after which polyamine transport kinetics were determined in agmatine-free conditions.