Activity | |
---|---|
% of control | |
p38α | 9.4 ± 2.81-150 |
p38γ | 93.7 ± 4.5 |
ERK-1 | 109.6 ± 6.8 |
JNK-2 | 102.9 ± 12.2 |
Protein kinase A | 101.0 ± 5.6 |
Protein kinase C | 90.5 ± 8.8 |
Protein kinase G | 79.6 ± 14.4 |
EGFR kinase | 107.2 ± 11.6 |
After purified kinases were preincubated with 10 μM FR167653 or vehicle (control) for 3 min, kinase reactions were initiated by adding myelin basic protein and [γ-32P]ATP. Five minutes later, the reactions were terminated, and the radioactivity incorporated into the protein substrate was determined. Data are presented as means ± S.E. (n = 4).
↵1-150 Significantly different from the control.