Table 1

Kinetic constants for the inactivation of P450s 1A1, 1A2, 2B1, and 2E1 by BITC in microsomes

P450	K_I	k_inact	t_1/2
	μM	min⁻¹	min
1A1	35	0.26	2.7
1A2	28	0.09	7.7
2B1	16	0.18	3.8
2E1	18	0.05	14

Assay conditions and the kinetic analyses were as described underMaterials and Methods. The kinetic constants were determined from double-reciprocal plots of the initial rates of inactivation (Fig.1) against BITC concentrations.