Treatment | Formation of Estradiol-Oleoyl Ester | |
---|---|---|
1 nM E2 | 50 μM E2 | |
pmol/mg protein/min | ||
Control diet | 0.02 ± 0.01 | 33 ± 6 |
0.60% clofibrate diet | 0.23 ± 0.01 | 510 ± 77 |
Female rats were fed an AIN-76A diet or 0.60% clofibrate in an AIN-76A diet for 4 weeks. [3H]Estradiol (1 nM) was incubated with 100 μM oleoyl-CoA and 0.25 mg of protein/ml for microsomes from control rats or 0.1 mg of protein/ml for microsomes from clofibrate-treated rats at 37°C for 10 min. The data for esterification of 50 μM estradiol were obtained from Fig. 9. Each value is the mean ± S.D. obtained from liver microsomes from three to four rats.