Fatty Acyl-CoA Incubated | Formation of Estradiol Fatty Acid Esters | ||
---|---|---|---|
Control | Gemfibrozil (300 mg/kg/day) | Clofibrate (400 mg/kg/day) | |
pmol/mg protein/min | |||
Palmitoyl-CoA (C16:0) | 30 ± 4 | 163 ± 17 | 190 ± 18 |
(443%↑) | (533%↑) | ||
Palmitoleoyl-CoA (C16:1) | 33 ± 5 | 128 ± 11 | 170 ± 5 |
(288%↑) | (415%↑) | ||
Stearoyl-CoA (C18:0) | 43 ± 12 | 240 ± 34 | 333 ± 32 |
(458%↑) | (674%↑) | ||
Oleoyl-CoA (C18:1) | 51 ± 6 | 283 ± 3 | 452 ± 6 |
(455%↑) | (786%↑) | ||
Linoleoyl-CoA (C18:2) | 40 ± 6 | 212 ± 14 | 297 ± 19 |
(430%↑) | (642%↑) | ||
Arachidonoyl-CoA (C20:4) | 44 ± 0.2 | 304 ± 11 | 404 ± 19 |
(591%↑) | (818%↑) |
Adult female rats were injected i.p. with gemfibrozil (300 mg/kg in corn oil), clofibrate (400 mg/kg in corn oil), or vehicle (corn oil) once daily for 4 days. The following day, the rats were sacrificed, and livers from four rats per group were pooled for the preparation of hepatic microsomes. The incubation mixture consisted of 1 mg/ml microsomal protein from control rats or 0.5 mg/ml microsomal protein from induced rats, 50 μM [4-14C]estradiol (E2), and 100 μM of each fatty acyl-CoA in a final volume of 0.5 ml of sodium acetate buffer (0.1 M, pH 5.0). Each value is the mean ± S.D. obtained from triplicate determinations with the same liver microsomal preparation. The percentage of increase over control values is indicated in parentheses.