Fatty Acyl-CoA Incubated | Formation of Estradiol Fatty Acid Ester |
---|---|
pmol/mg protein/min | |
Palmitoyl-CoA (C16:0) | 30.5 ± 0.5 |
Palmitoleoyl-CoA (C16:1) | 41.8 ± 0.9 |
Stearoyl-CoA (C18:0) | 41.2 ± 1.5 |
Oleoyl-CoA (C18:1) | 48.0 ± 1.0 |
Linoleoyl-CoA (C18:2) | 42.1 ± 2.3 |
Arachidonoyl-CoA (C20:4) | 63.0 ± 2.9 |
Liver microsomes (1 mg of protein/ml) from adult female rats were incubated for 30 min with 10 μM [4-14C]estradiol and 100 μM fatty acyl-CoA in a final volume of 0.5 ml of sodium acetate buffer (0.1 M, pH 5.0). Formation of estradiol fatty acid ester was measured as described under Experimental Procedures. Each value is the mean ± S.D. of triplicate determinations with the same liver microsomal preparation.