Primer | PCR Product1-a | Sequence1-b |
---|---|---|
Primers for PCR of the CYP2B1 gene | ||
Primer A (forward) | 2B1/12 kbp | 5′-ACCCATCCCCATAAAACAAG-3′ |
Primer B (reverse) | 2B1/12 kbp | 5′-TTTTCTCGAAGCTGCCAAAC-3′ |
Primer C (forward) | 2B1/6 kbp | 5′-TTAACAATTTCTGTGCTGGC-3′ |
Primer D (reverse) | 2B1/6 kbp | 5′-ATGGGGTACACTTCAGTGTT-3′ |
Primer E (forward) | 2B1/7 kbp | 5′-AAAAGAGGACAGAGACTACA-3′ |
Primer F (reverse) | 2B1/7 kbp | 5′-GAAGTAAAGGCACAAGACTC-3′ |
Primers for PCR of the CYP2B2 gene | ||
Primer G (forward) | 2B2/PBRU | 5′-GAACAGTGTGTTCTACTTTC-3′ |
Primer H (reverse) | 2B2/PBRU | 5′-TATGTAAATGGGTGTGCACT-3′ |
Primer I (forward) | 2B2/12 kbp | 5′-CTTTTTGTGATGTTCAGCCA-3′ |
Primer J (reverse) | 2B2/12 kbp | 5′-ATGGCATTTTGGTACGATCA-3′ |
Primer K (forward) | 2B2/4 kbp | 5′-TGATCGTACCAAAATGCCAT-3′ |
Primer L (reverse) | 2B2/4 kbp | 5′-TGAAGTAAAGGCACAACACA-3′ |
Primer M (forward) | 2B2/0.6 kbp | 5′-ACATGGGCACGAAGAAACAG-3′ |
Primer N (reverse) | 2B2/0.6 kbp | 5′-AGGATGGGATACACCTCAGT-3′ |
↵1-a See Fig. 1 for the location of the region amplified in the gene. Primers E and M were designed from the introns that were currently determined in this laboratory.
↵1-b Underlines indicate the different bases between CYP2B1 and CYP2B2 (Suwa et al., 1985; Hoffman et al., 1992; Shaw et al., 1996).