Drug-induced changes in Purkinje neuron membrane polarization (mV) of Sprague-Dawley (S-D), GEPR-NE, GEPR-3, and GEPR-9 rats
| Drug | Postnatal Days 10–14 | Postnatal Days 15+ | ||||||
|---|---|---|---|---|---|---|---|---|
| S-D | GEPR-NE | GEPR-3 | GEPR-9 | S-D | GEPR-NE | GEPR-3 | GEPR-9 | |
| Muscimol | −11.3 ± 0.4 | −10.8 ± 0.6 | −8.5 ± 0.42-150 , 2-160 | −5.0 ± 0.52-150 | −11.6 ± 0.2 | −11.3 ± 0.3 | −9.6 ± 0.32-150 , 2-160 | −5.2 ± 0.22-150 |
| (17) | (6) | (7) | (11) | (78) | (8) | (14) | (69) | |
| Muscimol + bicuculline | −0.3 ± 0.2 | 0.5 ± 0.6 | 0.5 ± 0.8 | 0.2 ± 0.3 | −0.1 ± 0.3 | −0.1 ± 0.5 | 0.4 ± 0.9 | 0.1 ± 0.2 |
| (3) | (6) | (4) | (3) | (9) | (6) | (5) | (7) | |
| GABA | −11.7 ± 0.7 | −10.3 ± 0.7 | −9.1 ± 0.42-150 , 2-160 | −3.7 ± 0.42-150 | −11.1 ± 0.2 | −11.1 ± 0.2 | −10.1 ± 0.32-150 , 2-160 | −4.8 ± 0.22-150 |
| (5) | (6) | (5) | (7) | (14) | (12) | (15) | (12) | |
| GABA + bicuculline | −0.1 ± 1.1 | 0.7 ± 0.5 | −1.0 ± 0.6 | 0.1 ± 0.7 | −1.2 ± 0.3 | −1.2 ± 0.3 | −0.6 ± 0.5 | −0.2 ± 0.3 |
| (3) | (5) | (5) | (6) | (4) | (8) | (5) | (5) | |
| Bicuculline | 0.6 ± 0.4 | 0.1 ± 0.5 | 0.1 ± 0.5 | −0.7 ± 0.3 | 1.0 ± 0.5 | 1.0 ± 0.5 | −0.1 ± 0.5 | 0.2 ± 0.2 |
| (3) | (4) | (4) | (4) | (6) | (6) | (6) | (8) | |
| Baclofen | −1.6 ± 0.2 | −0.3 ± 0.6 | 1.1 ± 0.62-150 | 1.3 ± 0.22-150 | −0.9 ± 0.2 | −0.9 ± 0.2 | 1.2 ± 0.62-150 | 0.3 ± 0.22-150 |
| (3) | (6) | (4) | (4) | (6) | (6) | (4) | (11) | |
| Glutamate | 12.8 ± 0.3 | 12.9 ± 0.6 | 12.2 ± 0.7 | 12.6 ± 0.4 | 16.7 ± 0.2 | 16.3 ± 0.3 | 16.3 ± 0.4 | 16.2 ± 0.2 |
| (7) | (5) | (5) | (6) | (9) | (8) | (8) | (12) | |
| Aspartate | 10.8 ± 0.4 | 11.6 ± 0.6 | 10.9 ± 0.6 | 11.7 ± 0.5 | 13.6 ± 0.2 | 13.2 ± 0.4 | 13.3 ± 0.4 | 13.1 ± 0.2 |
| (4) | (5) | (4) | (7) | (9) | (7) | (6) | (12) | |
Changes in membrane potential (mV) of Purkinje neurons in slices from Sprague-Dawley, GEPR-NE, GEPR-3, and GEPR-9 rats induced by application of a single concentration of drug during intracellular recording. Mean responses are reported in mV ± S.E.M. Numbers in parentheses represent the number of cells in the recording population from no less than three different preparations each from different litters. For both muscimol and GABA, only hyperpolarizing responses were used to calculate the mean response. All drug concentrations were 10 μM except for muscimol (1 μM) and bicuculline (30 μM).