Table 2

Biological activities of ligands and receptor constructs relative to the activities of each ligand at wild type receptors, the activities of natural ligands at each receptor construct, and action of natural ligands at wild type receptors

	Ligands
	Sec	S^1–15V^16–28	Lys¹⁵Sec	Met¹⁵Sec	S^1–14V^15–28	V^1–14S^15–27	Asp¹⁵VIP	Met¹⁵VIP	V^1–15S^16–27	VIP
Action relative to action at Sec receptor
Sec	1	1	1	1	1	1	1	1	1	1
Ve₂S	2	1	1	0.5	0.2	1	2	0.2	3	67
Se₁e₂V	2	7	22	37	50	0.2	0.1	1	0.2	53
Se₁V	22	3	6	1	1	43	5	1	5	36
Ve₁S	77	250	0.1	1	1	1	8	1	0.03	8
Se₂V	0.02	0.01	0.1	0.02	0.1	0.0004	0.002	0.1	0.01	0.1
VPAC₁	42	5	50	60	17	105	25	2	15	0.2
Action relative to action at VPAC₁ receptor
Sec	1	1	1	2	4	1	1	4	0.3	6
Ve₂S	1	0.1	0.04	0.03	0.02	5	7	2	5	1
Se₁e₂V	0	0.4	0.2	1	1	0.02	0.2	1	0.2	1
Se₁V	0	0.004	15	2	2	1	0.1	2	36	5
Ve₁S	53	83	20	63	15	2418	667	13	140	80
Se₂V	0	0.2	0.02	0.02	0.1	0.01	0.04	1	0.1	1
VPAC₁	1	1	1	1	1	1	1	1	1	1
Action relative to action of Sec at this receptor
Sec	1	1	15	1	1	63	1	0.4	7	1
Ve₂S	1	0.3	13	1	0.4	350	2	0.1	91	0.04
Se₁e₂V	1	1	41	1	1	2	1	0.2	33	0.3
Se₁V	1	0.2	89	0.2	0.4	91	0.1	0.1	125	0.04
Ve₁S	1	1	0.02	2	0.3	161	1	0.01	6	0.3
Se₂V	1	0.3	0.1	1	1	1	4	0.3	1	1
VPAC₁	1	0.04	1	1	0.4	6	1	0.02	4	0.1
Action relative to action of VIP at this receptor
Sec	2	0.1	1	1	0.02	1	3	0.1	1	1
Ve₂S	3	0.1	2	2	0.003	1	16	0.01	25	1
Se₁e₂V	1	0.02	1	2	1	1	4	0.03	4	1
Se₁V	7	0.01	6	3	0.01	8	17	0.01	28	1
Ve₁S	2	42	1	3	0.01	1	200	0.2	4	1
Se₂V	3	10	2	1	1	0.3	4	2	2	1
VPAC₁	28	1	2	3	0.2	1	53	0.2	15	1
Action relative to action of natural hormone at WT receptor
Sec	1	0.5	7.5	7.5	6.5	410	590	230	1665	1150
Ve₂S	1.5	0.5	6.5	3.5	1.5	525	890	55	5000	200
Se₁e₂V	3	3.5	145	130	75	115	130	30	995	265
Se₁V	65	10	885	145	55	5000	665	40	5000	180
Ve₁S	5000	2500	60	95	30	4835	5000	25	140	40
Se₂V	95	30	3	1.5	2	2	7.5	2	1	0.5
VPAC₁	4035	145	150	90	33.5	210	185	3.5	15	1