Compound | cAMP Elevation, EC200 | TNF-α Production, IC50 |
---|---|---|
nM | ||
YM976 | 12 (5.1–24) | 9.4 (5.4–16) |
Rolipram | 93 (14–306) | 76 (40–131) |
RP73401 | 1.1 (0.50–2.04) | 0.82 (0.50–1.3) |
SB207499 | NT | 120 (91–150) |
CDP840 | NT | 19 (12–27) |
cAMP elevation was evaluated under the stimulation by PGE2 (1 μM) in U937 cells. TNF-α production from human PBMC was induced by LPS (60 μg/ml). Each IC50 value was calculated from the percentage inhibition of control. Data are expressed as mean (95% confidence limits) of three to four separate experiments.
NT, not tested.