Effect of 12(R,S)-HETE, 12(S)-HETE, and 12(R)-HETE (0.3 μM) on unstimulated tissue and cAMP-dependent chloride secretion
| Isc | RTE | |||||
|---|---|---|---|---|---|---|
| Basal | Secretagogue | 12(R,S)-HETE | Basal | Secretagogue | 12(R,S)-HETE | |
| μA/cm2 | Ω · cm2 | |||||
| Unstimulated (n = 5) | 31.4 ± 2.6 | 24.1 ± 3.3* | 126.2 ± 27.0 | 130.5 ± 26.7 | ||
| DBcAMP (n = 8) | 9.3 ± 5.5 | 139.8 ± 12.0*** | 93.8 ± 12.1†† † | 155.7 ± 22.2 | 88.4 ± 5.0** | 94.5 ± 5.0** |
| PGE2 (n = 5) | 9.1 ± 6.3 | 87.2 ± 22.2* | 60.2 ± 18.1†† | 207.8 ± 23.9 | 116.7 ± 9.4* | 118.0 ± 9.3 |
| Theophylline (n = 10) | 28.0 ± 4.7 | 128.2 ± 15.6*** | 86.7 ± 10.5†† † | 137.5 ± 18.7 | 108.2 ± 13.8* | 110.9 ± 13.9 |
| 12(S)-HETE | 12(S)-HETE | |||||
|---|---|---|---|---|---|---|
| Theophylline (n = 6) | −3.6 ± 4.7 | 82.7 ± 8.8*** | 66.7 ± 5.6† | 142.0 ± 12.7 | 60.6 ± 4.3* | 57.8 ± 4.7 |
| 12(R)-HETE | 12(R)-HETE | |||||
|---|---|---|---|---|---|---|
| Theophylline (n = 5) | 8.1 ± 85 104.0 ± 4.8*** 93.8 8 ± 6.3†† 140.6 ± 8.2 105.4 ± 18.7* 106.6 ± 15.9 |
Significantly different from basal condition, *P < .05, **P < .01, ***P < .001.
Significantly different from secretagogue, †P< .05, ††P < .01,†††P < .001.