Percentage of Caco-2 cells of monolayers with normal microtubules
| Treatment | Normal |
|---|---|
| % | |
| Vehicle | 99 ± 1 |
| 2.5% EtOH | 72 ± 22-a |
| 15% EtOH | 27 ± 52-a |
| l-NIL alone | 97 ± 4 |
| l-NIL + 2.5% EtOH | 84 ± 22-b |
| l-NIL + 15% EtOH | 70 ± 42-b |
| Urate + 2.5% EtOH | 82 ± 62-b |
| Urate + 15% EtOH | 73 ± 72-b |
| l-Cysteine + 2.5% EtOH | 86 ± 32-b |
| l-Cysteine + 15% EtOH | 69 ± 52-b |
| d-Cysteine alone | 96 ± 2 |
| d-Cysteine + 2.5% EtOH | 71 ± 32-c |
| d-Cysteine + 15% EtOH | 22 ± 42-c |
| SOD + 2.5% EtOH | 85 ± 22-b |
| SOD + 15% EtOH | 70 ± 52-b |
| iSOD alone | 94 ± 3 |
| iSOD + 2.5% EtOH | 65 ± 52-c |
| iSOD + 15% EtOH | 24 ± 62-c |
Values are mean ± S.E. after treatments. Monolayers were exposed to EtOH for 30 min or were preincubated with 1) a selective iNOS inhibitor [l-NIL (1 mM)] or 2) ONOO− scavengers urate (0.5 mM) or l-cysteine (3 mM and, as control,d-cysteine) or SOD 300 U/ml and the heat-inactivated iSOD) for 30 min (except 1 h for l-NIL studies) before the exposure to EtOH. Controls were incubated in isotonic saline/vehicle. From 100 to 150 cells from monolayers per slide (well) were examined by high-resolution laser confocal and the percentage of cells displaying normal microtubule cytoskeleton was determined. n = 6 per group.