Treatment | FSA Clearance |
---|---|
nl/h/cm2 | |
Vehicle | 24 ± 11 |
2.5% EtOH | 296 ± 91-a |
15% EtOH | 2548 ± 1051-a |
l-Cysteine + 2.5% EtOH | 98 ± 81-b |
l-Cysteine + 15% EtOH | 911 ± 341-b |
d-Cysteine alone | 33 ± 7 |
d-Cysteine + 2.5% EtOH | 285 ± 81-a |
d-Cysteine + 15% EtOH | 2476 ± 401-a |
SOD + 2.5% EtOH | 102 ± 211-b |
SOD + 15% EtOH | 1205 ± 1351-b |
iSOD alone | 28 ± 10 |
iSOD + 2.5% EtOH | 279 ± 71-a |
iSOD + 15% EtOH | 2411 ± 2161-a |
Values are mean ± S.E. after treatments. Apical chambers were loaded with FSA before the exposure of monolayers to EtOH alone for 30 min or preincubation with ONOO− scavengers 1)l-cysteine (3 mM) and as control analogd-cysteine or 2) SOD (300 U/ml) and as control the heat-inactivated iSOD for 30 min before EtOH. Controls were incubated in isotonic saline/vehicle. FSA clearance was calculated as apical-to-basolateral flux of FSA divided by the concentration of probe in the apical chamber. n = 6 per group.