Composition of solutions used for saponin and Triton X-100 experiments
| Solution: Function (pCa) | [EGTA] | [Imidazole] | [Na+] | [Mg2+] | [Mg2+ATP] | [Phosphocreatine] | [Dithiothreitol] |
|---|---|---|---|---|---|---|---|
| mM | |||||||
| Basic solutions: relaxing and maximal activating (9.0 and 4.5) | 10 | 30 | 30.6 | 1 | 3.16 | 12 | 0.3 |
| Saponin experiment solutions | |||||||
| 1: depleting (9.0) | 10 | 30 | 30.6 | 1 | 3.16 | 12 | 0.3 |
| 2: washing (9.0) | 10 | 30 | 30.6 | 1 | 3.16 | 12 | 0.3 |
| 3: loading (7.0) | 10 | 30 | 30.6 | 1 | 3.16 | 12 | 0.3 |
| 4: washing (7.0 or 7.5) | 0.4 | 30 | 30.6 | 0.1 | 3.16 | 12 | 0.3 |
| 5: releasing (7.0 or 7.5) | 0.4 | 30 | 30.6 | 0.1 | 3.16 | 12 | 0.3 |
For saponin experiments, solution 1 contained 25 mM caffeine and solution 5 contained 0.1 to 25 mM caffeine or 2.5 μM to 2 mM 4-CmC.