Group | IL-8 Levels in Cultured CBMCs and Rectal Mucosa Medium | |||
---|---|---|---|---|
CBMCs from Normal Rats | CBMCs from TNB-Induced Colitis Rats | Rectal Mucosa from Normal Rats | Rectal Mucosa from TNB-Induced Colitis Rats | |
pg/ml | ||||
LPS (−) | 33.4 ± 17.0 | 81.9 ± 46.05-a | 53.5 ± 26.3 | 1560.4 ± 244.45-b |
LPS (100 μg/ml) | 1094.8 ± 277.4 | 1695.9 ± 243.85-c | 1925.1 ± 744.6 | 5125.5 ± 1640.25-b |
LPS (100 μg/ml) + Berberine (10−6M) | 1093.8 ± 240.7 | 1941.1 ± 581.6 | 2102.2 ± 927.3 | 4978.0 ± 970.6 |
LPS (100 μg/ml) + Berberine (10−5 M) | 1039.2 ± 257.6 | 1688.2 ± 485.3 | 1007.4 ± 214.85-d | 3321.4 ± 733.65-d |
Values are mean ± S.D. (n = 5). Berberine chloride (15 mg/kg) decreased IL-8 production of cultured rectal mucosa with stimulation of LPS compared with control group.