Stimulation of [35S]GTPγS binding by BU48 acting at recombinant δ-, μ-, or κ-opioid receptors
| EC50 | Maximal Effect3-a | |
|---|---|---|
| nM | % | |
| C6δ | 0.81 ± 0.27 | 39.7 ± 2.0 |
| C6μ | 0.085 ± 0.053 | 10.0 ± 3.3 |
| CHOκ | 0.78 ± 0.22 | 58.6 ± 6.6 |
Membranes from C6δ, C6μ, or CHO-hkor cells were incubated with [35S]GTPγS (0.1 nM), GDP (30 μM), and varying concentrations of BU48 for 60 min at 30°C as described underMaterials and Methods. Data represent means ± S.E. from three determinations.
↵3-a Percentage of response caused by a maximal (10 μM) concentration of SNC80 (C6δ), fentanyl (C6μ), or U69593 (CHOκ).