Mitogenesis of multiple cell types treated with LPA plus various RTKs
| Cell Type | RTK | RTK Alone | LPA + RTK | Response |
|---|---|---|---|---|
| VSM | None | 4.7 ± 0.4 (n = 3) | ||
| EGF | 2.5 ± 0.2 (n = 3) | 8.5 ± 0.6 (n = 3) | ADD | |
| bFGF | 1.4 ± 0.1 (n = 3) | 4.8 ± 0.1 (n = 3) | No RTK stimulation | |
| PDGF-BB | 9.4 ± 0.7 (n = 3) | 21.5 ± 2.0 (n = 3) | SYN | |
| HMC | None | 6.5 ± 0.4 (n = 3) | ||
| EGF | 2.3 ± 0.6 (n = 3) | 15.6 ± 4.0 (n = 3) | SYN | |
| bFGF | 4.2 ± 1.1 (n = 3) | 21.8 ± 5.5 (n = 3) | SYN | |
| PDGF-BB | 9.0 ± 2.3 (n = 3) | 45.7 ± 13.2 (n = 3) | SYN | |
| HFL-1 | None | 13.2 ± 1.2 (n = 8) | ||
| EGF | 7.0 ± 0.9 (n = 8) | 31.3 ± 5.5 (n = 8) | SYN | |
| bFGF | 15.4 ± 3.0 (n = 6) | 43.0 ± 10.8 (n = 6) | SYN | |
| IGF-1 | 1.4 ± 0.4 (n = 2) | 13.7 ± 0.9 (n = 2) | No RTK stimulation | |
| HFF | None | 5.7 ± 1.1 (n = 5) | ||
| EGF | 4.0 ± 0.4 (n = 5) | 11.3 ± 1.8 (n = 5) | ADD | |
| bFGF | 5.9 ± 1.2 (n = 5) | 13.7 ± 4.0 (n = 5) | ADD | |
| IGF-1 | 1.5 ± 0.2 (n = 5) | 6.0 ± 1.4 (n = 5) | No RTK stimulation | |
| 1321N1 | None | 4.4 ± 1.5 (n = 5) | ||
| EGF | 1.9 ± 0.6 (n = 5) | 3.8 ± 1.5 (n = 5) | <ADD |
[3H]Thymidine incorporation assays were performed with each cell type listed. Cells were treated with 10 μM LPA, 10 ng/ml bFGF, 60 ng/ml EGF, 10 ng/ml IGF-1, 20 ng/ml PDGF-BB, or combinations as indicated. Fold increases (means ± S.E.) are reported; the number of experiments, each performed in triplicate, is indicated in parentheses. Combinations were determined to be either synergistic (SYN), greater than additive (>ADD), additive (ADD), or less than additive (<ADD).