Table 2

Methodological citation for 5-HT receptor binding methods

ReceptorRadioligandBufferTissueNonspecificIncubateReference
nM mM h/°C
5-HT1A [3H]DPAT (2.0)Tris + salts2-a 50 mM, pH 7.4Transfected LM(tk−) cells5-HT (0.01)0.5 /37 Zgombick et al., 1991
5-HT1B [3H]5-HT (5.0)Tris + salts2-a 50 mM, pH 7.4Transfected LM(tk−) cells5-HT (0.01)0.5 /37 Zgombick et al., 1991
5-HT1D [3H]5-HT (5.0)Tris + salts2-a 50 mM, pH 7.4Transfected LM(tk−) cells5-HT (0.01)0.5 /37 Zgombick et al., 1991
5-HT1E [3H]5-HT (5.0)Tris + salts2-a 50 mM, pH 7.4Transfected LM(tk−) cells5-HT (0.01)0.5 /37 Zgombick et al., 1991
5-HT1F [3H]5-HT (5.0)Tris + salts2-a 50 mM, pH 7.4Transfected LM(tk−) cells5-HT (0.01)0.5 /37 Zgombick et al., 1991
5-HT2A [125I]DOI (0.5)Tris + salts2-a 50 mM, pH 7.4Transfected AV12 cells5-HT (0.01)0.5 /37 Zgombick et al., 1991
5-HT2B [3H]5-HT (10.0)Tris + salts2-b 50 mM, pH 7.4Transfected AV12 cells5-HT (0.01)0.5 /37 Kursar et al., 1994
5-HT2C [125I]DOI (0.5)Tris + salts2-a 50 mM, pH 7.4Transfected AV12 cells5-HT (0.01)0.5 /37 Zgombick et al., 1991
5-HT4 [3H]5-HT (5.0)Tris + salts2-a 50 mM, pH 7.4Transfected COS7 cells5-HT (0.01)0.5 /37 Zgombick et al., 1991
5-HT6 [3H]LSD (3.0)Tris + salts2-a 50 mM, pH 7.4Transfected HeLa cells5-HT (0.01)0.5 /37 Zgombick et al., 1991
5-HT7 [3H]5-HT (5.0)Tris + salts2-a 50 mM, pH 7.4Transfected COS7 cells5-HT (0.01)0.5 /37 Zgombick et al., 1991

  • ↵2-a  Salts: 10 mM MgCl2, 0.5 mM EDTA, 10 μM pargyline, and 0.1% ascorbate.

  • ↵2-b  Salts: 3 mM CaCl2, 0.5 mM EDTA, 10 μM pargyline, 0.1% ascorbate.